Yean-Chang Chen's Algal Home Page
Laboratory of algal applied physiology
The current research topics:
Immobilized microalgae:
Immobilization of twelve benthic diatom species for long-term storage and as feed for postlarval abalone Haliotis diversicolor. 2007. Aquaculture.263/1-4:97-106.
Immobilized
microalga Scenedesmus quadricauda (Chlorophyta, Chlorococcales) for
long-term storage and for application in fish culture water quality control.
2001. Aquaculture. 195(1-2):71-80 (SCI)
Immobilized Isochrysis galbana (Haptophyta) for long-term storage and applications for feed and water quality control in clam (Meretrix lusoria) cultures. 2003. J. Appl. Phycol. 15:439-444 (SCI)
Macroalgal protoplasts:
The published papers
Development
of protoplasts of Ulva fasciata
(Ulvales, Chlorophyta) for algal seed stock. 2000.
Development of protoplasts from holdfasts and vegetative thalli of Monostroma latissimum (Chlorophyta, Monostromataceae) for algal seed stock. 1998. J. Phycol. 34 (6): 1075-1081 (SCI)
Microbodies of protoplasts of Grateloupia sparsa (Halymeniaceae, Rhodophyta). 1996. Acta Oceanographica Taiwanica, 35: 201-210.
Ultrastructure of cell wall regeneration from isolated protoplasts of Grateloupia sparsa (Halymeniaceae, Rhodophyta). 1995.Bot. Mar. 38: 393-399. (SCI)
Isolation and regeneration of protoplasts of Monostroma latissimum Wittrock. 1994. Bot. Bull. Acad. Sin. 35: 47-53. (SCI)
Development of protoplasts from Grateloupia sparsa and G. filicina (Halymeniaceae, Rhodophyta). 1994. Bot. Mar. 37: 361-366. (SCI)
Use of fluorescent staining to monitor the temporal pattern of cell wall resynthesis in Ulva fasciata (Chlorophyta: Ulvales, Ulvaceae) protoplasts. 1993. J. of Jpn. Phycol. 41: 237-242.
The published papers concerning algal ultrastructure:
Development
of protoplasts of Ulva fasciata
(Ulvales, Chlorophyta) for algal seed stock. 2000.
The ultrastructure of Grateloupia filicina (Halymeniaceae, Rhodophyta) pit plugs. 1999. Bot. Mar. 42: 531-538 (SCI)
Ultrastructure
of cell wall regeneration from isolated protoplasts of Grateloupia
sparsa (Halymeniaceae, Rhodophyta). 1995.Bot. Mar. 38: 393-399. (SCI)
Microbodies of protoplasts of Grateloupia sparsa (Halymeniaceae, Rhodophyta). 1996. Acta Oceanographica Taiwanica, 35: 201-210.
Macroalgal
seed stock:
Left Figure: Cultivation of Grateloupia filicina filaments.
Right Figure: Cultivation of Monostroma latissimum filaments.
Filaments of Monostroma latissimum (Chlorophyta)
Filaments of Grateloupia filicina (Rhodophyta)
Microthalli (callus-like of thalli) of Ulva fasciata (Chlorophyta)
Immobilized
of microalgae:
Recently results:
Already entrapped
Scenedesmus quadricauda into alginate-beads and 
stored those beads in 4oC
at the darkness without any medium addition for more than 3 years. The beads
containing Scenedesmus quadricauda remain alive when re-suspended in the
medium.
(Above figure shown that the Scenedesmus quadricauda was immobilized in alginate-beads, subsequently packaged in a nylon net bag)
Left figure shown that the bags were hanged in the fish cultures for controlling the water quality.
Applied immobilized Isochrysis galbana for clam (Meretrix lusoria) culture.
The pure cultures of microalgal species in Lab.:
Haptophyta
Prymnesiophyceae (Haptophyceae)
Isochrysis galbana
Chromophyta
Bacillariophyceae
pennate diatom
Amphora sp. (freshwater)
Amphora sp. (seawater)
centric diatom
Skeletonema costatum
Eustimatales
Nannochloropsis oculata
Blue-greens
Synechococcus sp. (seawater)
Lyngbya sp. (soil-air)
Spirulina platensis
Spirulina sp.
(Right Figure: Tank culture of Spirulina platensis)
Chlorophyta
Prasinophyceae
Tetraselmis elliptica
Chlorophyceae
Scenedesmus quadricauda
Haematococcus pluvialis
Chlorella pyrenoidosa
Chlorella ellipsoidea
Chlamydomonas reinhardtii
Oocytis sp.
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Yean-Chang Chen, Ph.D.
Associate Professor,
Department of Aquaculture,
National Taiwan Ocean University,
Keelung, Taiwan
Email: ycchen@mail.ntou.edu.tw
Tel: 886 2 24622192 ext. 5221
Fax: 886 2 24633150==============================================