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海洋大學養殖系藻類應用生理實驗室

（一般海水加富營養及淡水培養基專用配方 PG-SW/FW medium）

陳衍昌 製作

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Stock 1（巨量元素）公克/升

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NaNO₃                                        xx/L dH₂O

NaH₂PO₄ • 2H₂O                       xx

NH₄Cl                                         xx

Na₂EDTA                                   xx

FeCl₃ • 6H₂O                             xx

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Stock 2（微量元素）

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CuSO₄ • 5H₂O                        0.x g/L dH₂O

ZnSO₄ • 7H₂O                        0.0x

CoCl₂ • 6H₂O                        0.0x

MnCl₂ • 4H₂O                       0.xx

NaMoO₄ • 2H₂O                   0.0xxx

Biotin                                    0.0xx

Vitamin B₁₂                          0.0xx

Thiamin HCl（Vitamin B₁）                        0.x

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Stock 3 for fresh water（淡水培養基需另加 1 mL之H₃BO₃＋MgSO₄ • 7H₂O stock sol.）

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H₃BO₃                             x.xx g/L dH₂O

MgSO₄ • 7H₂O                xx g/L

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 Stock 4 for diatom（培養矽藻需另加 1 mL之Na₂SiO₃ • 9H₂O stock sol.）

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Na₂SiO₃ • 9H₂O                        xx g/L dH₂O

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  Stock 5 for seaweed（培養海水大型藻需另加 1 mL之GeO₂ stock sol.）

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GeO₂                        x g/L dH₂O

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最終使用法（The final working medium）

　

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每升海水添加 Stock 1及2, 各1 mL，如為矽藻再加Stock 4, 1 mL，如為大型藻改加Stock 5, 1 mL。

每升淡水添加 Stock 1,2及3, 各2 mL，如為矽藻再加Stock 4, 1 mL。

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藻類應用生理實驗室（Spirulina配方）

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Zarrouk^註 modified medium（Z9配方）

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H₃PO₄                           xx mL/L of water

NaHCO₃                    xx g /L of water

KNO₃                        xx

K₂SO₄                        x

NaCl                           x

MgSO₄ • 7H₂O           x

CaCl₂                         x

FeSO₄                      xxxx

EDTA                       xxxx

plus 1 milliliter each of solutions A and B for each liter of the above.

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Solution A

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H₃BO₃                               xxx g/L of water

MnCl₂ • 4H₂O                    xxx

ZnSO₄ • 7H₂O                    xxx

CuSO₄ • 5H₂O                    xxx

MoO₃                                  xx

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Solution B

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NH₄VO₃                                  ??? x 10^-4 g /L of water

K₂Cr(SO₄)₄ • 24H₂O               ??? x 10^-4

NiSO₄ • 6H₂O                          ??? x 10^-4

Na₂WO₄ • 2H₂O                      ??? x 10^-4

Co(NO₃)₂ • 6H₂O                    ??? x 10^-4

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註：Zarrouk, C.（1966）博士論文