Molecular Biology & Endocrinology Laboratory

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President

National Taiwan Ocean University

National Chair Professor Ching-Fong Chang

 

I. General Information

 

Present Position                           President (National Taiwan Ocean University, NTOU)

                                                        Distinguished Professor (National Taiwan Ocean University, NTOU)

                                                        National Chair Professor (Ministry of Education)

                                                       

 

Address                                          Department of Aquaculture

                                                         National Taiwan Ocean University (NTOU)

                                                         Keelung, 20224

                                                         Taiwan

Telephone

Tel (office)                                 +886-2-2462-2110、+886-2-2462-2192      Ext 1000~1004(O), 5209(L)

Fax                                             +886-2-2463-3563(O)、+886-2-2462-1579(O)

Email                                         B0044@email.ntou.edu.tw

Education

Ph.D.               1986                  Department of Animal Sciences

                                                        Washington State University

                                                        Pullman, WA, USA

 

M.S.                1982                  Department of Animal Sciences

                                                        Washington State University

                                                        Pullman, WA, USA

 

B.S.                 1975                  Department of Marine Food Science

                                                        National Taiwan Ocean University

                                                        Keelung, Taiwan

Teaching Courses

         Undergraduate level:

               Fish Physiology

               Lab of Fish Physiology

               General Biology (Physiology section)

         Graduate level:

                Endocrinology, Fish Reproductive Physiology

Research area

         Reproductive Biology (fish, prawns, corals)

         Endocrine Physiology (fish, prawns, corals)

         Stress Physiology and immunity (fish)

         Sex Differentiation (fish)

         Molecular biology

Previous experience

        Department chairperson, Department of Aquaculture, National Taiwan Ocean University (NTOU) (1986/08-1992/07)

        Dean of Academic Affairs, National Taiwan Ocean University (2003-2006)

        Director. Center of Excellence of Bioenvironment and Biotechnology (2006-2007, 2010-2011)       

        Director General, Department of Life Science, National Science Council, Taiwan (2007/08/01-2009/07/31)

        Vice President, National Taiwan Ocean University (2010/08/01-2011/04/30)

        Deputy Minister, National Science Council, Taiwan (2011/5/1-2012/5/19) 

        President, Asia and Oceania Society of Comparative Endocrinology (2017-2020)

        Chairman, Taiwan Marine Biotechnology Society (2016/12/26-2018/12/25)

 

II. Honors and Awards

Excellent Research Award

1990

National Science Council, Taiwan

Honor for as an "Outstanding Young Person" at Keelung city

1991

Junior Chamber at Keelung, Taiwan

Distinguished Research Award

1991-1993

National Science Council, Taiwan

Honor for as "Ten Outstanding Young Persons" in Taiwan, R.O.C.

1993

Junior Chamber, Taiwan

Distinguished Research Award

1994-1996

National Science Council, Taiwan

Distinguished Research Award

1996-1998

National Science Council, Taiwan

Award for as a Special Research Fellow

1998-2000

National Science Council, Taiwan

Outstanding teacher in college of life sciences

2000

National Taiwan Ocean University

Award for as a Special Research Fellow

2001-2004

National Science Council, Taiwan

Award as a Distinguished Professor

2004

National Taiwan Ocean University

Academic Award

2004

Ministry of Education, Taiwan

Distinguished Special Research Fellow

2004

National Science Council

National Chair Professor Award

2006

Ministry of Education

National Taiwan Ocean University Distinguished Alumni

2007

National Taiwan Ocean University

Distinguished teaching award

2010

National Taiwan Ocean University

Taiwan-France Science and Technology Award (2012 awarded jointly by National Science Council and French Academy of Science)

2012

National Science Council, Taiwan

CAAPS 2013 Distinguished Professional Award

2013

Chinese American Academic & Professional Society

Distinguished Contribution Award

2013

The Chinese-Taipei Society of Laboratory Animal Sciences

Biology Science
Distinguished Research Award

2013

The Biological Society of China

Outstanding Alumnus Award

2014

Department of Animal Science

Washington State University

Shinkishi Hatai Medal Award

2016

Pacific Science Association of Japan

 

 

III. The Research and Teaching Activities

We have been conducting a series of experiments related to “fish reproductive physiology”, “sex differentiation and sex change in fish”, “reproductive endocrinology and reproduction in coral”, “hormone regulation in fish”, “maturation mechanism in fish”, “environmental physiology and immunity in fish”, and “reproductive physiology in prawns” in the past 20 years at the National Taiwan Ocean University. The researches are focusing on the marine animals especially important aquaculture species (black porgy and grouper).

The most significant contribution to the scientific community is the studies of “reproductive biology and physiology in aquatic animals” (as a representative work). A series of the experiments were conducted such as sex differentiation, sex change mechanism, regulation of reproductive hormones, gonadal development and growth, gametogenesis, maturation, and reproduction control. We have many important new findings and significant contributions to the theory and applied aspects in the area of reproductive physiology. We applied biological methods, histology, physiology, endocrinology, cell biology, and molecular genetics to solve the scientific questions and problems; most importantly, we further provided the new concepts and new proposals on the basis of our experimental results. Our research has earned the respect by scientific colleagues from Taiwan and international levels. The lab had been recognized as the important and famous location in the world-wide to work on sex change, coral research and other related research.

In the past 20 years, I also spent much time in the teaching activities. The courses (General Biology, Fish Phsiology, Endocrinology, Fish Reproduction) were taught in the Department of Aquaculture. These courses are important in the area of aquatic life sciences. The students ranked these courses are one of the best in their curriculum. The students have developed strong background after finishing these courses.

Ⅳ. Publication list

1.

Jeng, S. S., Wang, C. M., and Chang, C. F. 1978.  Existence of an estrogen-like compound in the ovary of the shrimp Parapenaeus fissurus.  Gen. Comp. Endocrinol. 36, 211-214.  (SCI)

2.

Chang, C. F., and Estergreen, V. L.1983. Development of a direct enzyme immunoassay of milk progesterone and its application to pregnancy diagnosis in cows. Steroids. 41, 173-195.  (SCI)

3.

Wimpy, T. H., Chang, C. F., Estergreen, V. L., and Hillers, T. K. 1986. Milk progesterone enzyme immunoassay: modifications and a field trial for pregnancy detection in dairy cows. J. Dairy Sci. 69, 1115-1121.  (SCI)

4.

De Silva, M., Tjoelker, L., Chang, C. F., and Reeves, J. J. 1986.  Ovarian response to active immunization against luteinizing hormone in the cow. Theriogenology. 26, 89-100.  (SCI)

5.

Chang, C. F., McFarland, S. Y., and Reeves, J. J. 1987.  Active immunization of heifers against prostaglandin F2αPotential as a sterilization vaccine. J. Anim. Sci. 64, 1090-1098.  (SCI)

6.

Chang, C. F. and Reeves, J. J. 1987.  Postpartum interval in beef cows shortened by enclomiphene. J. Anim. Sci. 65, 217-223.  (SCI)

7.

Chang, C. F., Robert, A. J., and Reeves, J. J. 1987.  Increased luteinizing  hormone secretion and ovarian function in heifers actively immunized against estrogen and progesterone. J. Anim. Sci. 65, 771-776.  (SCI)

8.

Johnson, H. E., DeAvila, D. M., Chang, C. F., and Reeves, J. J. 1988. Active immunization of heifers against Luteinizing Hormone-releasing hormone, human chorionic gonadotropin and bovine luteinizing hormone. J. Anim. Sci. 66, 719-726.  (SCI)

9.

Robert, A. J., Chang, C. F., Schally, A. V., and Reeves, J. J. 1989. Luteinizing hormone concentrations in serum of postpartum beef cows injected with microencapsulated luteinizing hormone releasing hormone analog. J. Anim. Sci. 67, 2730-2734.  (SCI)

10.

Reeves, J. J., Chang, C. F., deAvila, D. M., Grieger, D. M., Johnson, H. E., and Roberts, A. J. 1989.  Vaccine against endogenous hormones: a possible future tool in animal production. J. Dairy Sci. 72, 3363-3371.  (SCI)

11.

Chang, C. F., and Yueh W. S. 1989. Gonadal histology and steroidal levels in the male protandrous black porgy.  In "Current Topics in Marine Biotechnology". Edited by S. Miyachi, I. Karube and Y. Ishida. pp. 327-332. Fuji Technology Press Ltd. Tokyo. Japan.

12.

Chang, C. F., and Chen, M. R. 1989. The distribution and characteristic of steroid-binding protein in fish and shrimp. In "Current Topics in Marine Biotechnology". Edited by S. Miyachi, I. Karube and Y. Ishida. pp. 333-336. Fuji Technology Press Ltd. Tokyo. Japan.

13.

Yueh, W. S., Lin, S. F., and Chang, C. F. 1990. Effects of LHRH-A and HCG on spermiation volume, concentration of milt and sex steroids in black porgy, Acanthopagrus schlegeli. J. Fish Soc. Taiwan. 17, 65-72.

14.

Chang, C. F., and Chen, M. R. 1990. The difference in the degree of steroid-binding activity in fishes. Bull. Inst. Zool., Academia Sinica. 29, 41-47.  (SCI)

15.

Chang, C. F., and Yueh, W. S. 1990. Oocyte maturation in protandrous black porgy, Acanthopagrus  schlegeli, stimulated by enclomiphene and luteinizing hormone releasing hormone analogue. Bull. Inst. Zool., Academia Sinica. 29, 173-179.  (SCI)

16.

Chang, C. F., and Chen, M. R.  1990. Fluctuation in sex steroids and sex steroid-binding protein during the development and annual cycle of the male common carp, Cyprinus  carpio. Comp. Biochem. Physiol. 97A, 565-568.  (SCI)

17.

Chang, C. F., and Yueh, W. S. 1990. Annual cycle of gonad histology and steroid profiles in the juvenile males and adult females of the protandrous black porgy, Acanthopagrus schlegeli. Aquaculture. 91, 179-196.  (SCI)

18.

Chang, C. F., Yueh, W. S., and Lee, M. F. 1991. Effects of LHRH-A and HCG on the steroid profiles of bisexual and mature male and female protandrous black porgy, Acanthopagrus schlegeli. Aquaculture. 92, 83-92.  (SCI)

19.

Chang, C. F. and Chen, M. R. 1991. Lack of correlation found between plasma sex steroids and sex steroid-binding capacity during the annual cycle of the female common carp. Bull. Inst. Zool., Academia Sinica. 30, 81-86.  (SCI)

20.

Chang, C. F. and Lin, S. J. 1991. An enkephalin analog stimulates growth of tilapia. Fish Physiol. Biochem. 9, 101-106.  (SCI)

21.

Chang, C. F., Hu, H. J., Tang, H. C., and Sun, L. T.1992. Stimulation of ovulation in ayu, Plecoglossus  altivelis, by treatment with antiestrogens and luteinizing hormone releasing hormone analog. Aquaculture. 101, 329-336.  (SCI)

22.

Chang, C. F., Hu, H. J., and Tang, H. C. 1992. Effects of photoperiod and temperature on testicular development in male ayu, Plecoglossus altivelis. Environ. Biol. Fishes. 34, 309-314.  (SCI)

23.

Sun, L. T., Hu, H. J., Tang, H. C., Huang, C. F., and Chang, C. F. 1992. Changes of sex steroid concentrations in female and male ayu  (Plecoglossus altivelis)  stimulated by  luteinizing hormone releasing hormone analog. Bull. Inst. Zool., Academia Sinica 31, 57-64.  (SCI)

24.

Chang, C. F. and Lee, Y. H. 1992. Purification of the sex steroid- binding protein from common carp plasma. Comp. Biochem. Physiol. 101B, 587-590.  (SCI)

25.

Sun, L. T., Chen, G. R., and Chang, C. F. 1992. The Physiological responses of tilapia exposed to low temperatures. J. Therm. Biol. 17, 149-153.  (SCI)

26.

Lee, M. F., Chang, C. F., Huang, J. D., and Schally, A. V. 1993. Microencapsulated LHRH analog accelerates spermiation in protandrousblack porgy, Acanthopagrus  schlegeli. Bull. Inst. Zool., Academia Sinica. 32, 32-38.  (SCI)

27.

Chang, C. F., Lee, F. Y., and Huang, Y. S. 1993. Purification and characterization of vitellin from the mature ovaries of prawn, Penaeus monodon. Comp. Biochem. Physiol. 105B, 409-414.  (SCI)

28.

Chang, C. F., Shih, T. W., and Hong, H. H. 1993. Purification and characterization of vitellin from the mature ovaries of prawn, Macrobrachium rosenbergii. Comp. Biochem. Physiol. 105B, 609-615.  (SCI)

29.

Chang, C. F., Hu, H. J., and Sun, L. T.  1993. Effects of temperature and photoperiod on ovarian development in female ayu, Plecoglossus altivelis. J. Therm. Biol. 18, 197-201.  (SCI)

30.

Tsai H. J., Chi, W. K., Chang, C. C., Kao, T. T., and Chang, C. F. 1993. Enhacement of tilapia growth by dietary administration of recombinant yeast lysates as a supplement. J. Fish Soc. Taiwan 20, 339-345.

31.

Sun L. T., Chen, G. R., and Chang, C. F. 1994. Characteristics of blood parameters and gill Na+-K+-ATPase in chilled comatose tilapia cultured in various salinities. Comp. Biochem. Physiol. 107A, 641-646.  (SCI)

32.

Chang, C. F., Lee, M. F., and Chen, G. R. 1994. Estradiol-17Β associated with the sex reversal in protandrous black porgy, Acanthopagrus schlegeli. J. Exp. Zool. 268, 53-58.  (SCI)

33.

Chang, C. F., Lee, Y. H., Yoshida, T., and Sun, L. T. 1994. Characterization of the plasma sex steroid-binding protein in eel  (Anguilla japonica) . Comp. Biochem. Physiol. 108B189-197.  (SCI) .

34.

Chang, C. F., Lee, F. Y., Huang, Y. S., and Hong, T. H. 1994. Purification and characterization of the female-specific protein  (vitellogenin)  in mature female hemolymph of prawn, Penaeus monodon. Invert. Reprod. Dev. 25, 185-192.  (SCI)

35.

Lin, S. J., Tin, Y. Y. and Chang, C. F. 1994. The stimulatory effects of 3,5,3'-triiodo- L-thyronine on the content of liver soluble protein in tilapia. J. Fish Soc. Taiwan. 21, 361-368.

36.

Chang, C. F. and Shih, T. W. 1995. Reproductive cycle of ovarian development and vitellogenin profiles in the freshwater prawns, Macrobrachium rosenbergii. Invert. Reprod. Dev. 27, 11-20.  (SCI)

37.

Chang, C. F. and Lin, S. J. 1995. Immersion in bovine insulin stimulates growth of tilapia. Reprod. Nutr. Dev. 35, 95-103.  (SCI)

38.

Chang, C. F., Sun, L. T. and Chen, G. R. 1995 Acute responses of blood parameters and comatose effects in salt-acclimated tilapia exposed to low temperatures. J. Therm. Biol. 20, 299-306.  (SCI) .

39.

Chang, C. F., Yueh, W. S., Lee, M. F. and Schally A. V. 1995. A microencapsulated analog of LHRH accelerates maturation but without stimulating sex reversal in the protandrous black porgy, Acanthopagrus schlegeli. Reprod. Nutr. Dev. 35, 339-349.  (SCI)

40.

Chen, G. R., Sun, L. T., Lee, Y. H. and Chang, C. F.1995. Characteristics of blood in common carp  (Cyprinus carpio)  exposed to low temperatures. J. Applied Aquaculture 5, 21-31.

41.

Chang, C. F., Lan, S. C., and Chou, H. Y. 1995. Gonadal histology and plasma sex steroids during sex differentiation in grey mullet, Mugil cephalus. J. Exp. Zool. 272, 395-406.  (SCI)

42.

Chang, C. F., and Jeng S. R. 1995.  Isolation and Characterization of the female-specific protein  (vitellogenin)  in mature female hemolymph of the prawn, Penaeus chinensis. Comp. Biochem. Physiol. 112B, 257-263.  (SCI)

43.

Chang, C. F., Lau, E. L., and Lin B. Y. 1995. Estradiol-17β suppresses testicular development and stimulates sex reversal in protandrons black porgy, Acanthopagrus schlegeli. Fish Physiol. Biochem. 14, 481-488.  (SCI)

44.

Chang, C. F., Lan, S. C., and Pan, P. S. 1995. Feed administration of estradiol-17β stimulates female differentiation in juvenile grey mullet, Mugil cephalus. Zool. Stud. 34, 257-264.  (SCI)

45.

Chang, C. F., Lau, E. L., and Lin, B. Y.  1995. Stimulation of spermatogenesis or of sex reversal according to the dose of exogenous estradiol-17β in juvenile males of protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol. 100, 355-367.  (SCI)

46.

Chang, C. F., Lau, E. L., Lin, B. Y., and Jeng, S. R. 1996. Characterization of vitellogenin induced by estradiol-17β in protandrons black porgy, Acanthopagrus schlegeli. Fish Physiol. Biochem. 15, 11-19.  (SCI)

47.

Chang, C. F., Jeng, S. R., Lin, M. N., and Tin, Y. Y. 1996. Purification and characterization of vitellin from the mature ovaries of prawn, Penaeuschinensis. Invert. Reprod. Dev. 29, 87-93.  (SCI)

48.

Lee F. Y., and Chang, C. F. 1997. The concentrations of vitellogenin  (vitellin)  and protein in hemolymph, ovary and hepatopancreas in different ovarian stages of the freshwater prawns, Macrobrachium rosenbergii. Comp. Biochem. Physiol.117A, 433-439.  (SCI)

49.

Yueh, W. S., and Chang, C. F. 1997. 17a, 20β, 21- Trihydroxy-4- pregnen-3-one and 17a, 20β-dihydroxy-4-pregnen-3-one stimulate spermiation in protandrous black porgy, Acanthopagrus schlegeli.  Fish Physiol. Biochem. 17, 187-193.  (SCI)

50.

Lau, E. L., Lin, B. Y., Lee, F. Y., Sun, L. T., Dufour, S., and Chang, C. F. 1997.  Stimulation of testicular function by exogenous testosterone in male protandrous black porgy, Acanthopagrus schlegeli. J. Fish Biol. 51, 327-333.  (SCI)

51.

Huang, Y. S., Schmitz, M., Le Belle, N., Chang, C. F., Querat, B., and Dufour, S. 1997. Androgens stimulate gonadotropin-II b subunit in the eel pituitary cells in vitro. Mol. Cell Endocrinol.131, 157-166.  (SCI)

52.

Chang, C. F., Lin, B. Y., Lau, E. L., Lee, M. F., Yueh, W. S., Chang, C. N., Lee, Y. H., Tacon, P., Lee, F. Y., Du, J. L., and Sun, L. T. 1997. The endocrine mechanism of sex reversal in the protandrous black porgy, Acanthopagrus schlegeli: a review. Chin. J. Physiol. 40, 197-205.  (SCI)

53.

Lee, F. Y, Shih, T. W., and Chang, C. F. 1997. Isolation and characterization of the female specific protein  (vitellogenin)  in mature female hemolymph of the freshwater prawn, Macrobrachium rosenbergii: comparison with ovarian vitellin. Gen. Comp. Endocrinol. 108, 406-415.  (SCI)

54.

Chang, C. F. and Lin, B. Y. 1998. Estradiol-17β stimulates aromatase activity and reversible sex change in protandrous black porgy, Acanthopagrus schlegeli. J. Exp. Zool. 280, 165-173.  (SCI)

55.

Huang, Y. S., Le Belle, N., Schmitz, M., Chang, C. F., Vernier, P., Querat, B., and Dufour, S. 1998. In vivo and in vitro effects of sex steroids on gonadotropin  (GtH-II)  synthesis in the female European eel. Ann. New York Academy Sci. 839, 348-350.  (SCI)

56.

Chang, C. F., Hung, C. Y., Chiang, M. C., and Lan, S, C. 1999. The concentrations of plasma sex steroids and gonadal aromatase during controlled sex differentiation in grey mullet, Mugil cephalus. Aquaculture 177, 37-45.  (SCI)

57.

Lee, F. Y., and Chang, C. F. 1999. Hepatopancreas is the likely organ of vitellogenin synthesis in the freshwater prawn, Macrobrachium rosenbergii. J. Exp. Zool. 284, 798-806.  (SCI)

58.

Lee, Y. H., Du, J. L., Yueh, W. S., Lee, F. Y., Tanaka, H., and Chang, C.F. 1999. 17b–Estradiol, but not testosterone stimulates gonadotropin II concentrations in the protandrous black porgy, Acanthopagrus schlegeli Bleeker. Fish Physiol. Biochem. 21, 345-351.  (SCI)

59.

Yueh W. S., and Chang, C. F. 2000. Morphological changes and competence of the maturing oocytes in the protandrous black porgy, Acanthopagrus schlegeli. Zool. Stud. 39, 114-122.  (SCI)

60.

Lee, Y. H., Lee, F. Y., Yueh, W. S., Tacon, P., Du, J. L., Chang, C. N., Jeng, S. R. , Tanaka, H., and Chang, C. F. 2000. Profiles of gonadal development, sex steroids, aromatase activity and gonadotropin II in the controlled sex changes of protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol. 119, 111-120.  (SCI)

61.

Tsai, C. L., Wang, L. H., Chang, C. F., and Kao, C. C. 2000. Effects of gonadal steroids on brain serotonergic and aromatase activity during the critical period of sexual differentiation in tilapia, Oreochromis mossambicus. J. Neuroendocrinol. 12, 894-898.  (SCI)

62.

Jeng, S. R., Chen, G. R., Lai, J. Y., Huang, Y. S., Dufour, S., and Chang, C. F. 2000. Sex steroids and pituitary extract increase gonadotropin II content in the pituitary of aquacultured Japanese eel Anguilla japonica. J. Fish. Res. 9: 97-108.

63.

Du. J. L., Lee, C. Y., Tacon, P., Lee, Y. H., Yen, F. P., Tanaka, H., Dufour, S. and Chang, C. F. 2001. Estradiol-17b stimulates gonadotropin II expression and release in the protandrous male black porgy, Acanthopagrus schlegeli Bleeker: A possible role in sex change. Gen. Comp. Endocrinol. 121, 135-145.  (SCI)

64.

Law, W. Y., Chen, W. H, Song, Y. L., Dufour, S., and Chang, C. F. 2001. Differential in vitro suppressive effects of steroids on the leukocyte phagocytosis in tilapia and common carp. Gen. Comp. Endocrinol. 121, 163-172.  (SCI)

65.

Lee, Y. H., Du, J. L., Yen, F. P., Lee, C. Y., Dufour, S., Sun, L. T. and Chang, C. F. 2001. Regulation of plasma gonadotropin II secretion by sex steroids, aromatase inhibitors and antiestrogens in the protandrous black porgy, Acanthopagrus schlegeli Bleeker. Comp. Biochem Physiol. 129B, 399-406.  (SCI)

66.

Lee, Y. H., Du, J. L., Yueh, W. S., Lin, B. Y., Huang, J. D., Lee, C. Y., Lee, M. F., Lau, E. L., Lee, F. Y., Morrey, C., Nagahama, Y., and Chang, C. F. 2001. Sex change in the protandrous black porgy, Acanthopagrus schelgeli: a review in gonadal development, estradiol, estrogen receptor, aromatase activity and gonadotropin. J. Exp. Zool. 290, 715-726.  (SCI)

67.

Yen, F. P., Lee, Y. H., He, C. L., Huang, J. D., Sun, L. T., Dufour, S., and Chang, C. F. 2002. Estradiol-17b triggers luteinizing hormone release in the protandrous black porgy, Acanthopagrus schlegeli Bleeker through multiple interactions with GnRH control. Biol. Reprod. 66, 251-257.  (SCI)

68.

Jeng, S. R., Chen, G. R., Lai, J. Y., Huang, Y. S., Dufour, S., and Chang, C. F.  2002 Regulation of pituitary gonadotropin II and growth hormone content by sex steroids and pituitary extract in the aquacultured Japanese eel, Anguilla japonica. Aquaculture 209, 319-332.  (SCI)

69.

Chen, W. H., Sun L. T., Tsai, C. L., Song, Y. L., and Chang, C. F. 2002. Cold-stress induced the modulation of the catecholamines, cortisol, immunoglobulin M, and leukocyte phagocytosis in tilapia. Gen. Comp. Endocrinol. 126, 90-100.  (SCI)

70.

Lee, Y. H., Yueh, W. S., Du, J. L., Sun, L. T., and Chang, C. F. 2002. Aromatase inhibitors block natural sex change and induce male function in the protandrous black porgy, Acanthopagrus schlegeli Bleeker: Possible mechanism of natural sex change. Biol. Reprod. 66, 1749-1754.  (SCI)

71.

Yueh, W. S., and Chang, C. F. 2002. Oocyte maturation-inducing steroids in protandrous black porgy, Acanthopagrus schlegeli. Comp. Biochem. Physiol. 131C, 345-353.  (SCI)

72.

Huang, J. D., Lee, M. F., and Chang, C. F. 2002. The morphology of gonadal tissue and male germ cell in the protandrous black porgy, Acanthopagrus schlegeli. Zool. Stud. 41, 216-227.  (SCI)

73.

Huang, Y. S., Yueh, W. S., Huang, J. D., Du, J. L., Sun, L. T., Nagahama, Y., and Chang, C. F. 2002. Cloning and expression of estrogen receptors in the protandrous black porgy, Acanthopagrus schlegeli: the implication of sex change mechanism. Mar. Biotechnol. 4, 236-246.  (SCI)

74.

Lo, W. Y., Chang, C. F., and Song, Y. L. 2003. Evaluation of dorsal aorta cannulation for immunological studies of grouper  (Epienphelus malabaricus) . Fish Shellfish Immunol. 14, 289-303.  (SCI)

75.

Yeh, S. L., Kuo, C. M., Ting, Y. Y., and Chang, C. F. 2003. The effects of exogenous androgens on ovarian development and sex change in female orange-spotted protogynous grouper, Epinephelus coioides. Aquaculture 218, 729-739. (SCI)

76.

Twan, W. H., Hwang, J. S., and Chang, C. F. 2003. Sex steroids in Scleractinian coral, Euphyllia ancora: The implication in mass spawning. Biol. Reprod. 68, 2250-2260  (SCI)

77.

He, C. L., Du, J. L., Huang, Y. S., Lee, Y. H., Nagahama, Y., and Chang, C. F. 2003. Differential mRNA transcription of androgen receptor and estrogen receptor in gonad in relation to the sex change in protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 69, 455-461.  (SCI)

78.

Yeh, S. L., Dai, Q. C., Chu, Y. T., Kuo, C. M., Ting, Y. Y., and Chang, C. F. 2003. Induced sex change, spawning and larviculture of potato grouper, Epinephrine tukula. Aquaculture 228, 371-381  (SCI)

79.

Yeh, S. L., Chu, Y. T., Kuo, C. M., Ting, Y. Y., and Chang, C. F. 2003. 17a–methyltestosterone induced sex change in the unilateral ovariectomized protogynous orange-spotted grouper, Epinephelus coioides. J. Fish. Soc. Taiwan 30, 91-100.

80.

Yeh, S. L., Kuo, C. M., Ting, Y. Y., and Chang, C. F. 2003. Androgen stimulated sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males. Comp. Biochem. Physiol. 135C, 375-382.  (SCI)

81.

Du, J. L., Lin, B. Y., Lee, Y. H., Yueh, W. S., Huang, J. D., Lee, M. F., Sun, L. T., and Chang, C. F. 2003. The possible mechanism of sex change in the protandrous black porgy, Acanthopagrus schlegeli. J. Fish Soc. Taiwan 30, 101-119.

82.

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He, C. L., Du, J. L., Wu, G. C., Lee, Y. H., Sun, L. T., and Chang, C. F. 2003. Differential Dmrt1 transcripts in gonads of the protandrous black porgy, Acanthoparus schlegeli. Cytogenet. Genome Res. 101: 309-313. (SCI)

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Du, J. L., Lin, B. Y., Lee, Y. H., Yueh, W. S., He, C. L., Lee, M. F., Sun, L. T., and Chang, C. F. 2003. Estradiol, aromatase and steroid receptors involved in the sex change of protandrous black porgy, Acanthopagrus schlegeli. Fish Physiol. Biochem. 28, 131-133. (SCI)

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Lee, Y. H., Wu, G. C., Du, J. L., and Chang, C. F. 2004. Estradiol-17b induced a reversible sex change in the fingerlings of protandrous black porgy, Acanthopagrus schlegeli Bleeker. Biol. Reprod. 71, 1270-1278.  (SCI)

86.

Lee, Y. H., Du, J. L., Shih, Y. S., Sun, L. T. , and Chang, C. F. 2004. In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone secretion in the protandrous black porgy, Acanthopagrus schlegeli Bleeker. Gen. Comp. Endocrinol.  139,12-19.  (SCI)

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Wu, G. C., Du, J. L., Lee, Y. H., Lee, M. F., and Chang, C. F. 2005. Current status of genetic and endocrine factors in the sex change of protandrous black porgy, Acanthopagrus schlegeli. Annals New York Academy Sci. 1040, 206-214. (SCI)

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Yueh, W. S., Thomas, P., and Chang, C. F. 2005. 17,20b,21-Trihydroxy-4-pregnen-3-one as an oocyte maturation-inducing steroid in protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol.   140, 184-191. (SCI)

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Du, J. L., Lee, Y. H., Yueh, W. S., and Chang, C. F. 2005. Seasonal profiles of brain and pituitary GnRH and plasma LH in relation to sex change of protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 72, 922-931. (SCI)

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Jeng, S. R., Yueh, W. S., Lee, Y. H., Dufour, S., and Chang, C. F. 2005. Differential expression in neural and gonadal aromatase activity in relation to the reproductive activity in Japanese eel, Anguilla japonica. J. Exp. Zool. 303A, 802-812. (SCI)

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Twan, W. H., Wu, H. F., Hwang, J. S., Lee, Y. H., and Chang, C. F. 2005. Corals already have evolved the vertebrate-type hormone system in the sexual reproduction. Fish Physiol. Biochem. 31, 11-115  (SCI).

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Twan, W. H., Hwang, J. S., Lee, Y. H., Yueh, W. S.,Tung, Y. S., Wu, H. F., Dufour, S., and Chang, C. F. 2006. The presence and ancestral role in reproduction of GnRH-like in scleractinian coral, Euphyllia ancora. Endocrinology. 147, 397-406. (SCI)

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Twan, W. H., Hwang, J. S., Lee, Y. H., Wu, H. F., Tung, Y. S., and Chang, C. F. 2006. Homones and reproduction in scleractinian corals. Comp. Biochem. Physiol. 144A,  247-253. (SCI).

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Cheng, A. C., Chen, C. Y., Liou, C. H.and Chang, C. F. 2006. The effects of dietary protein and lipid in the blood parameters and superoxide anion production in grouper. Zool. Stud. 45, 492-502. (SCI).

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Twan, W. H., Lee, Y. H., Wu, H. F., Yueh, W. S., Jeng, S. R., Hwang, J. S. and Chang, C. F. 2007. Conserved roles of hormones in the reproduction of the scleractinian coral Euphyllia ancora. J. Fish. Soc. Taiwan 34, 1-9.

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Tomy, S., Wu, G. C., Huang, H. R., Dufour, S. and Chang, C. F. 2007. Developmental expression of key steroidogenic enzymes in the brain of protandrous black porgy fish, Acanthopagrus schlegeli. J Neuroendocrinol. 19, 643-655. (SCI).

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Jeng, S. R., Yeun, W. S., Chen, G. R., Lee, Y. H., Dufour, S.and Chang, C. F. 2007. Differential expression and regulation of gonadotropins and their receptors in the Japanese eel, Anguilla japonica. Gen. Comp. Endocrinol. 154, 161-173. (SCI).

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Wu, G. C., Tomy, S., and Chang, C. F. 2008. The expression of nr0b1 and nr5a4 during gonad development and sex change in protandrous black porgy fish, Acanthopagrus schlegeli. Biol. Reprod. 78, 200 - 210. (SCI)

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Lee, M. F., Huang, J. D., and Chang, C. F. 2008. The Development of Ovarian Tissue and Female Germ Cells in the Protandrous Black Porgy Fish, Acanthopagrus schlegeli. Zool. Stud. 47, 302-316. (SCI).

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Wu, G. C., Tomy, S., Nakamura, M., and Chang, C. F. 2008. Dual roles of cyp19a1a in gonadal sex differentiation and development in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 79, 1111-1120. (SCI).

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Tomy, S., Chang, Y. M., Chen, Y. H., Cao, J. C., Wang, T. P., and Chang, C. F. 2009. Salinity effects on the expression of osmoregulatory genes in the euryhaline black porgy Acanthopagrus schlegeli. Gen. Comp. Endocrinol.  161, 123-132. (SCI)

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Tomy, S., Wu, G. C., Huang, H. R. and Chang, C. F. 2009.  Age-dependent differential expression of genes involved in steroid signalling pathway in the brain of protandrous black porgy, Acanthopagrus schlegeli.  Dev. Neurobiol. 69, 299-313. (SCI)

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Wu, G. C. and Chang, C. F. 2009. Wnt4 is associated with the development of ovarian tissue in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 81, 1073-1082. (SCI)

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Aroua, S., Rousseau, K., Schmitz, M., Chang, C. F., and Dufour, S. 2009. The gonadoliberin(s)-gonadotropin(s) axis in the eel: expression and regulation under induced maturation and sex steroid feedbacks. In "Spawning Migration of the European Eel" (G. van den Thillart, S. Dufour & C. Rankin, eds), Springer, Fish and Fisheries Series, Vol 30, Chaper 11, pp 253-278.

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Guiguen, Y., Fostier, A., Pierrer, F., and Chang, C. F. 2010. Ovarian aromatase and estrogens: a pivotal role for gonadal sex differentiation and sex change in fish. Gen. Comp. Endocrinol. 165, 352-366. (SCI)

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Wu, G. C., Tomy, S., Lee, Y. H., Lee, M. F., Yueh, W. S., Lin, C. J., Lau, E. L. and Chang, C. F. 2010. Sex differentiation and sex change in the protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol. 167, 417-421 (SCI)

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Lin, C. J., Wu, G. C., Lee, M. F., Lau, E. L., Dufour, S., and Chang, C. F.  2010. Regulation of two forms of gonadotropin-releasing hormone receptor gene expression in the protandrous black porgy fish, Acanthopagrus schlegeli. Mol. Cell. Endocrinol. 323, 137-146. (SCI)

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Wu, G. C., Chiu, P. C., Lyu, Y. S., and Chang, C. F. 2010. The expression of amh and amhr2 is associated with the development of gonadal tissue and sex change in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 83, 443-453. (SCI)

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Lee, M. F., Huang, J. D. and Chang, C. F. 2011. Development of the genital duct system in the protandrous black porgy, Acanthopagrus schlegeli. Anat. Rec. 294, 494-505.(SCI)

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Tsai, Y. j., Lee, M. F., Chen, C. Y. and Chang, C. F. 2011. Development of gonadal tissue and aromatase function in the protogynous orange-spotted grouper, Epinephelus coioides. Zool. Stud. 50, 693-704. (SCI)

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Nagarajan, G., Tsai, Y. J., Chen, C. Y. and Chang, C. F. 2011. Developmental expression of genes involved in neural estrogen biosynthesis and signaling in the brain of the orange-spotted grouper Epinephelus coioides during gonadal sex differentiation. J. Steroid Biochem. Mol. Biol. 127, 155-166. (SCI)

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Chan, I., Tseng, C., Ka, S., Chang, C. F. and Hwang, J. S. 2012. An experimental study of the gorgonian coral Suborgorgia suberosa response to polluted sea water from a former mining site in Taiwan. Zool. Stud. 51, 27-37. (SCI)

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Arous, S., Maugars, G., Jeng, S. R., Chang, C. F., Welttzien, F. A., Rousseau, K., and Dufour, S. 2012. Pituitary gonadotropins FSH and LH are oppositely regulated by the activin/follistatin system in a basal teleost, the eel. Gen. Comp. Endocrinol. 175, 82-91. (SCI)

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Wu, G. C., Chiu, P. C., Lin, C. J., Lyu, Y. S., Lan, D. S., Chang, C. F. 2012. Testicular dmrt1 is involved in the sexual fate of the ovotestis in the protandrous black porgy. Biol. Reprod. 86: 41, 1-11. (SCI)

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Jeng, S. R., Pen, Y. T., Gueguen, M. M., Yueh, W. S., Dufour, S., Chang, C. F.*, and Kah, O.* 2012. Expression of aromatase in radial glial cells in the brain of Japanese eel provides insight into evolution of the cyp191a gene in Actinopterygians.  PLoS ONE. 7(9):e44750 (SCI). *: co-correspondence authors (Chang, CF and Kah, O.)

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Jeng, S. R., Pasquier, J., Yueh, W. S., Chen, G.R., Lee, Y. H., Dufour, S., and Chang, C. F.  2012. Differential regulation of the expression of cytochrome P450 aromatase, estrogen and androgen receptor subtypes in the brain-pituitary-ovarian axis of the Japanese eel (Anguilla japonica) reveals steroid dependent and independent mechanisms. Gen. Comp. Endocrinol. 175, 163-172. (SCI)

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Aruna, A., Nagarajan, G., Chang, C. F. 2012. Involvement of corticotropin-releasing hormone and corticosteroid receptors in the brain-pituitary-gill of tilapia during the course of seawater acclimation. J. Neuroendocrinol. 24, 818-830. (SCI)

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Jeng, S. R., Yueh, W. S., Lee, Y. H. Yen, H. F. and Chang, C. F.  2012. 17,20b,21-Trihydroxy-4-pregnen-3-one biosynthesis and 20b-hydroxysteroid dehydrogenase expression during final oocyte maturation in the protandrous yellowfin porgy, Acanthopagrus latus.  Gen. Comp. Endocrinol. 176, 192-200. (SCI)

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Shikina, S., Chen, C. J., Liou, J. Y., Shao, Z. F., Chung, Y. J., Lee, Y. H. and Chang, C. F. 2012. Germ cell development in the scleractinian coral Euphyllia ancora(Cnidaria, Anthozoa). PLoS ONE 7(7) : e41569. (SCI)    

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Dasquier, J., Lafont, A. G., Jeng, S. R., Morini, M., Dirks, R., Thillart, G. V. D., Tomiewicz, J., Tostivint, H., Chang, C. F., Rousseau, K., and S. Dufour. 2012. Multiple kisspeptin receptors in early osteichthyans provide new insight into evolution of this receptor family. PLoS ONE 7(11):e48931.(SCI)

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Aruna, A., Nagarajan, G., and Chang, C. F. 2012. Differential expression patterns and location of glucocoticoid and mineralocorticoid receptor transcripts in the osmoregulatory organs of tilapia during salinity stress. Gen. Comp. Endocrinol. 179, 465-476. (SCI)

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Chan, I., Peng, A. H., Chang, C. F., Hwang, J. S. and Hung, J. J. 2013. Effects of acidified seawater on the skeleton structure of the scleractinian coral, evidence identified by SEM. Zool. Stud. 51(8), 1319-1331. (SCI)

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Wu, G. C. and Chang, C. F. 2013.The switch of secondary sex determination in protandrous black porgy, Acanthopagrus schlegeli. Fish Physiol. Biochem. 39(1), 33-38. (SCI)

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Nagarajan, G., Aruna, A., and Chang, C. F. 2013.Neurosteroidogenic enzymes and their regulation in the early brain of the protogynous grouper Epinephelus coioides during gonadal sex differentiation. Gen. Comp. Endocrinol. 181, 271-287. (SCI)

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Nagarajan, G., Aruna, A., and Chang, C. F. 2013. Increase of estrogen signaling in the early brain of orange-spotted grouper Epinephelus coioides: A mini-review. Fish Physiol. Biochem. 39, 95-101. (SCI)      

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Wu, G. C. and Chang, C. F. 2013. Oocytes Survive in the Testis By Altering the Soma Fate from Male to Female in the Protandrous Black Porgy, Acanthopagrus schlegeli. Biol. Reprod. 88 (1) 19, 1-10.(SCI)

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Lau, E. L., Lee, M. F., and Chang, C. F. 2013. Conserved sex-specific timing of meiotic initiation during sex differentiation in the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod. 88(6) 150, 1-13. (SCI)

128.

Shikina, S., Chen, C. J., Chung, Y. J., Shao, Z. F., Liou, J. Y., Tseng, H. P., Lee, Y. H. and Chang, C. F. 2013. Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in non-bilaterian animals. Endocrinology 154, 3447-3459. (SCI)

129.

Jeng, S. R., Yueh, W. S., Pen, Y. T., Lee, Y. H., Chen, G. R., Dufour, S., and Chang, C. F. 2014. Neuroendocrine gene expression reveals a decrease in dopamine D2B receptor with no changes in GnRH system during prepubertal metamorphosis of silvering in wild Japanese eel. Gen. Comp. Endocrinol. 206, 8-15. (SCI)

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Aruna, A., Nagarajan, G., and Chang, C. F. 2015. The acute salinity changes activate the dual pathways of endocrine responses in the brain and pituitary of tilapia. Gen. Comp. Endocrinol. 211, 154-164. (SCI)

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Shikina S., Chung, Y. J., Wang, H. M., Chiu, Y. L., Shao, Z. F., Lee, Y. H., Chang, C. F. 2015. Localization of early germ cells in a stony coral, Euphyllia ancora: potential implications for a germline stem cell system in coral gametogenesis. Coral Reefs. 34, 639-653. (SCI)

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Wu, G. C., Li, H. W., Luo, J. W., Chen, C., and Chang, C. F. 2015. The potential role of Amh to prevent ectopic female development in testicular tissue of the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod. 92:158, 1-13. (SCI)

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Nagarajan, G, Aruna, A, Chang, C. F. 2015. Neuropeptide arginine vasotocin positively affects neurosteroidogenesis in the early brain of grouper, Epinephelus coioides. J. Neuroendocrinol. 27, 718-736. (SCI)

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Shikina S., Chiu, Y. L., Lee, Y. H., Chang, C. F. 2015. From somatic cells to oocytes: a novel yolk protein produced by the ovarian somatic cells in a stony coral, Euphyllia ancora. Biol. Reprod. 93(3)57, 1-10. (SCI)

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Wu, G. C., Trey, W. G., Li, H. W., and Chang, C. F. 2015. Sexual fate reprogramming in the steroid-induced bi-directional sex change in the protogynous orange-spotted grouper, Epinephelus coioide. PLoS ONE 10(12): e0145438. (SCI)

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Shikina, S and Chang, C. F. 2015. Sexual reproduction of stony coral: gametogenesis and spawning of Euphyllia ancora. Aquabiology. 219: 419-425. (in Japanese)

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Lin, C. J., Fan-Chiang, Y. C., Dufour, S., and Chang, C. F. 2016. Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, Acanthopagrus schlegelii. Dev. Neurobiol. 76:2, 121-136. (SCI)

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Chen, C. J., Shikina, S., Chen, W. J., Chung, Y. J., Chiu, Y. L., Bertrand, J. A. M., Lee, Y. H., and Chang, C. F. 2016. A novel female-specific and sexual reproduction-associated dmrt gene discovered in the stony coral, Euphyllia ancora. Biol. Reprod. 94(2)40, 1-13. (SCI)

139.

Shikina, S., Chung, Y. J., Chiu, Y. L., Huang, Y. J., Lee, Y. H., and Chang, C. F. 2016. Molecular cloning and characterization of a steroidogenic enzyme, 17β-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa). Gen. Comp. Endocrinol. 228, 95-104. (SCI)

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Wu, G. C., Li, H. W., Huang, C. H., Lin, H. J., Lin, C. J., and Chang, C. F. 2016. The testis is a primary factor that contributes to epigenetic modifications in the ovaries of the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod. 94(6):132, 1-13. (SCI)

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Shikina, S., Chiu, Y. L., Chung, Y. J., Chen, C. J., Lee, Y. H., and Chang, C. F. 2016. Oocytes express an endogenous red fluorescent protein in a stony coral, Euphyllia ancora: a potential involvement in coral oogenesis. Sci. Rep. 6: 25868. (SCI)

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Shikina, S. and Chang, C. F. 2016. Gametogenesis of reef-building corals. Biological Science. 67: 216-227. (in Japanese)

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Shikina, S. and Chang, C. F. 2016. Sexual reproduction in stony corals and insight into the evolution of oogenesis in Cnidaria. A chapter in The Cnidaria, past, present and future: The world of Medusa and her sisters. Stefano Goffredo, Zvy Dubinsky (eds). Chapter 16, 249-268. Springer.

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Wu, G. C. and Chang, C. F. 2017. Primary males guide the femaleness through the regulation of testicular Dmrt1 and ovarian Cyp19a1a in protandrous black porgy. Gen. Comp. Endocrinol. (doi: 10.1016/j.ygcan32017.01.033)  (SCI)

 145. Jeng, S. R., Wu, G. C., Yueh, W. S., Kuo, S. F., Dufour S., and Chang, C. F. 2017. Gonadal development and expression of sex-specific genes during sex differentiation in the Japanese eel. Gen. Comp. Endocrinol. (doi.org/10.1016/j.ygcen.2017.07.031) (SCI)
 146. Wu, G. C., Li, H. W., Tey, W. G., Lin, C. L., and Chang, C. F. 2017. Expression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides. PLoS ONE (doi.org/10.1371/journal.pone.0185864) (SCI)
 147. Wu, G. C., Luo, J. W., Li, H. W., Huang, C. H., and Chang, C. F. 2017. Robust gdf9 and bmp15 expression in the oocytes of ovotestes through the Figla-independent pathway in the hermaphroditic black porgy, Acanthopagrus schlegelii. PLoS ONE ((doi.org/10.1371/journal.pone.0186991) (SCI)
   

 

 

 

 

 

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